Chin J Dent Res 2026;29(3):227–235; doi:10.3290/j.cjdr.b7093222
Characterisation of Innate Lymphoid Cells in Human and Murine Periodontitis
Writer:Wen Min ZENG, Zong Xian LI, Chao ZHONG, Yu Feng XIE, Xiao Qian YU Clicked:
Objective: To evaluate the use of oral health services, the economic burden of oral diseases and related influential factors in China. Method: Using the multistage, stratified, equal proportion, random sampling method in the 4th National Oral Health Survey of China conducted in 2015 to 2016, residents aged 3 to 5 years, 12 to 15 years, 35 to 44 years, 55 to 64 years, and 65 to 74 years respectively were recruited, clinically examined and answered a questionnaire. Utilisation of oral health services and the economic burden of oral diseases in the past 12 months were assessed in all the age groups. Chi-squared tests, t tests, correlation analysis and a one-way ANOVA were used to determine the relationships between the different factors which were screened from the clinical examination and questionnaires with utilisation of oral health services and the economic burden of oral diseases. Results: In the subject groups 3 to 5 years (5876), 12 to 15 years (27,936) and 35 to 74 years (2708) the prevalence of the utili
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Objective: To characterise the subset dynamics of innate lymphoid cells (ILCs) in human and murine periodontitis to elucidate their roles in disease progression.
Methods: Human gingival samples were harvested from periodontitis patients and healthy controls. A murine ligature-induced periodontitis model was established. Gingival ILC subsets (ILC1s, ILC2s, ILC3s) were analysed by flow cytometry.
Results: Human periodontitis exhibited elevated total ILCs (from 1.34% to 2.06%) and a pronounced increase in ILC3s (from 0.92% to 29.70%). In mice, 3 days post-ligation, there was a marked decrease in total ILCs (from 10.80% to 0.85%), primarily due to the depletion of ILC2s (from 81.55% to 60.08%), whereas ILC1s (from 1.41% to 15.07%) and ILC3s (from 0.75% to 2.87%) expanded. These changes became even more pronounced 7 days after ligation.
Conclusion: Species-specific ILC dynamics underscore distinct immunopathogenic mechanisms: human periodontitis is characterised by the predominance of ILC3s, whereas, in murine disease, there is an association with the loss of ILC2s and an expansion of pro-inflammatory ILC1s/ILC3s. These observations place ILC3s as central mediators in chronic human diseases and emphasise the necessity for therapeutic strategies tailored to target specific ILC subsets.
Keywords: innate immunity, innate lymphoid cells, murine ligature-induced periodontitis, periodontitis
(editor:CJDR) |
